11/20/2022 0 Comments Rotor gene 6000 software free download![]() ![]() ![]() In case of subgenus Leishmania, the discrimination between L. HRM data obtained with qPCR-ML allowed to discriminate between subgenera Viannia and Leishmania. The qPCR assays (qPCR-ML and qPCR-ama), as well as ITS1 PCR, were performed directly from a punch of filter paper, with a pre-amplification step. The qPCR runs were analyzed with Rotor-Gene software version 1.7 to obtain Cq values and High resolution melting temperatures.ĭNA extracted from clinical samples and strains was spotted on filter paper for storage and laboratory transfer. The qPCR assays were performed using Rotor-Gene 6000 instrument (Corbett life science). Despite the limited number of clinical samples, these data are encouraging for a potential application in regions where L. The qPCR data were in agreement with the species characterization obtained with other methods such as conventional species-specific PCR, ITS1 PCR-RFLP or DNA sequencing. ![]() The Leishmania species has been determined in 27 canine samples and 11 human samples, exploiting HRM analysis of qPCR-ML and Cq values of qPCR-ML and qPCR-ama, as reported previously. The data are related to the article “Real-time PCR to differentiate among Leishmania ( Viannia) subgenus, Leishmania ( Leishmania) infantum and Leishmania ( Leishmania) amazonensis: application on Brazilian clinical samples”, and include also details of clinical evaluation/diagnosis of human patients and primer sequences used in the qPCR assays. This article contains the data regarding Leishmania species identification in human and canine clinical samples from a Brazilian region endemic for Leishmania ( Viannia) spp., Leishmania ( Leishmania) infantum and Leishmania ( Leishmania) amazonensis, using a previously developed approach involving two qPCR assays (qPCR-ML and qPCR-ama). ![]()
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